![]() ![]() Two dimensional en face dual-channel phase images are taken over 150 mm x 200 mm region on the microscopic slide and the images are reconstructed by plotting relative phase variation as the OCT beam is moved across the artery cross section. Microscopic slide for the tissue section is processed by standard histology method with mounting media. The lumen is cut open and sliced at 5 mm thickness. ![]() Another piece of skin from the same porcine ear sample with an area of 1.5 cm × 1.5cm was placed next to the initial sample as the control. The skin was flattened and pressed against the window to eliminate air gaps. Porcine renal artery is freshly harvested from a local slaughter house. A piece of the porcine ear skin with an area of 2cm × 2cm was placed onto the quartz win-dow of the THz-TDS system. Because phase noise in the environment is equally present in both channels, computation of phase difference between the two signal channels is attributed exclusively to variation in the specimen's composite refractive index. Two orthogonal polarization modes of light are spatially separated using a Wollaston prism and directed into separate photoreceivers. ![]() DP-OCT instrumentation is an all-fiber, dual channel Michelson interferometer constructed using polarization maintaining fiber. DP-OCT provides a quantitative measure of thin specimen phase retardation and refractive index with phase resolution of 5 nm and lateral resolution of 3 mm. Different techniques for measurement of refractive index of tissues are discussed. Refractive index data of biological tissues is reviewed and summarized. We describe a methodology to record spatial variation of refractive index of porcine renal artery using differential phase optical coherence tomography (DP-OCT). Download scientific diagram Refractive index profiles in the equatorial planes of three representative porcine (a),c) and e)) and three representative murine (b),d) and f)) lenses. Refractive index- a characteristic optical variable- controls light propagation in tissues. ![]()
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